<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "https://jats.nlm.nih.gov/nlm-dtd/publishing/3.0/journalpublishing3.dtd">
<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="research-article" dtd-version="3.0" xml:lang="en">
<front>
<journal-meta>
<journal-id journal-id-type="publisher">SD</journal-id>
<journal-title-group>
<journal-title>Scientific Drilling</journal-title>
<abbrev-journal-title abbrev-type="publisher">SD</abbrev-journal-title>
<abbrev-journal-title abbrev-type="nlm-ta">Sci. Dril.</abbrev-journal-title>
</journal-title-group>
<issn pub-type="epub">1816-3459</issn>
<publisher><publisher-name>Copernicus Publications</publisher-name>
<publisher-loc>Göttingen, Germany</publisher-loc>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.5194/sd-17-13-2014</article-id>
<title-group>
<article-title>IODP Expedition 336: initiation of long-term coupled microbiological, geochemical, and hydrological experimentation within the seafloor at North Pond, western flank of the Mid-Atlantic Ridge</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Edwards</surname>
<given-names>K.</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Bach</surname>
<given-names>W.</given-names>
</name>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Klaus</surname>
<given-names>A.</given-names>
</name>
<xref ref-type="aff" rid="aff3">
<sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>the IODP Expedition 336 Scientific Party</surname>
<given-names></given-names>
</name>
</contrib>
</contrib-group><aff id="aff1">
<label>1</label>
<addr-line>University of Southern California, California, USA</addr-line>
</aff>
<aff id="aff2">
<label>2</label>
<addr-line>University of Bremen, Bremen, Germany</addr-line>
</aff>
<aff id="aff3">
<label>3</label>
<addr-line>International Ocean Discovery Program, Texas A&amp;M University, Texas, USA</addr-line>
</aff>
<pub-date pub-type="epub">
<day>29</day>
<month>04</month>
<year>2014</year>
</pub-date>
<volume>17</volume>
<fpage>13</fpage>
<lpage>18</lpage>
<permissions>
<copyright-statement>Copyright: &#x000a9; 2014 K. Edwards et al.</copyright-statement>
<copyright-year>2014</copyright-year>
<license license-type="open-access">
<license-p>This work is licensed under the Creative Commons Attribution 3.0 Unported License. To view a copy of this licence, visit <ext-link ext-link-type="uri"  xlink:href="https://creativecommons.org/licenses/by/3.0/">https://creativecommons.org/licenses/by/3.0/</ext-link></license-p>
</license>
</permissions>
<self-uri xlink:href="https://sd.copernicus.org/articles/17/13/2014/sd-17-13-2014.html">This article is available from https://sd.copernicus.org/articles/17/13/2014/sd-17-13-2014.html</self-uri>
<self-uri xlink:href="https://sd.copernicus.org/articles/17/13/2014/sd-17-13-2014.pdf">The full text article is available as a PDF file from https://sd.copernicus.org/articles/17/13/2014/sd-17-13-2014.pdf</self-uri>
<abstract>
<p>Integrated Ocean Drilling Program (IODP) Expedition 336 addressed questions concerning
subseafloor microbial life and its relation to seawater circulation and basalt–seawater
reactions in the basaltic ocean crust. Sediment and basement samples were recovered at
three drill sites located in the North Pond area, an 8 &amp;times; 15 km large sediment
pond on the 8 Ma western flank of the Mid-Atlantic Ridge around 22°45&apos; N and
46°05&apos; W in roughly 4450 m water depth. The average core recovery rate
in basement was approx. 31%. The subseafloor depth of the basement holes ranges from
90 to 332 m; sediment thickness is between 36 and 90 m. Two of the holes (U1382A, and U1383C)
were equipped with advanced Circulation Obviation Retrofit Kit (CORK) observatories,
employing – for the first time – fiberglass casing. Another CORK string was deployed
in Deep Sea Drilling Project (DSDP) Hole 395A, but the wellhead broke off upon final installment.
Nonetheless, the North Pond observatory is fully operational and post-cruise observatory
research is already underway. Combined geochemical and microbiological studies of
the drill core samples and experimental CORK materials will help understand
(1) the extent and activity of microbial life in basalt and its relation to basalt
alteration by circulating seawater, and (2) the mechanism of microbial inoculation
of an isolated sediment pond.</p>
</abstract>
<counts><page-count count="6"/></counts>
</article-meta>
</front>
<body/>
<back>
<ref-list>
<title>References</title>
<ref id="ref1">
<label>1</label><mixed-citation publication-type="other" xlink:type="simple">Bartetzko, A., Pezard, P., Goldberg, D., Sun, Y.-F., and Becker, K.: Volcanic stratigraphy of DSDP/ODP Hole 395A: An interpretation using well-logging data, Mar. Geophys. Res., 22, 111–127, 2001.</mixed-citation>
</ref>
<ref id="ref2">
<label>2</label><mixed-citation publication-type="other" xlink:type="simple">Becker, K., Malone, M. J., et al.: Proc. ODP, Init. Repts., 174B: College Station, TX (Ocean Drilling Program), &lt;a href=&quot;http://dx.doi.org/10.2973/odp.proc.ir.174b.1998&quot;&gt;https://doi.org/10.2973/odp.proc.ir.174b.1998&lt;/a&gt;, 1998.</mixed-citation>
</ref>
<ref id="ref3">
<label>3</label><mixed-citation publication-type="other" xlink:type="simple">Becker, K., the Leg 174B Scientific Party, and Davis, E. E.: Leg 174B revisits Hole 395A: logging and long-term monitoring of off-axis hydrothermal processes in young oceanic crust, JOIDES J., 24, 1–3, 1998.</mixed-citation>
</ref>
<ref id="ref4">
<label>4</label><mixed-citation publication-type="other" xlink:type="simple">Cowen, J. P., Giovannoni, S. J., Kenig, F., Johnson, H. P., Butterfield, D. A., Rappe, M. S., Hutnak, M., and Lam, P.: Fluids from aging ocean crust that support microbial life, Science, 203, 120–123, 2003.</mixed-citation>
</ref>
<ref id="ref5">
<label>5</label><mixed-citation publication-type="other" xlink:type="simple">Cowen, J. P., Copson, D. A., Jolly, J., Hsieh, C.-C., Lin, H.-T., Glazer, B. T., and Wheat, C. G.: Advanced instrument system for real-time and time-series microbial geochemical sampling of the deep (basaltic) crustal biosphere, Deep-Sea Res. Pt. I, 61, 43–56, 2012.</mixed-citation>
</ref>
<ref id="ref6">
<label>6</label><mixed-citation publication-type="other" xlink:type="simple">Edwards, K. J., Becker, K., and Colwell, R. K.: The deep, dark energy biosphere: Intraterrestrial life on Earth, Annu. Rev. Earth Pl. Sc., 40, 551–568, 2012a.</mixed-citation>
</ref>
<ref id="ref7">
<label>7</label><mixed-citation publication-type="other" xlink:type="simple">Edwards, K. J., Fisher, A. T., and Wheat, C. G.: The deep subsurface biosphere in igneous ocean crust: frontier habitats for microbial exploration, Front. Microbiol, 3, &lt;a href=&quot;http://dx.doi.org/10.3389/fmicb.2012.00008&quot;&gt;https://doi.org/10.3389/fmicb.2012.00008&lt;/a&gt;, eCollection, 2012b.</mixed-citation>
</ref>
<ref id="ref8">
<label>8</label><mixed-citation publication-type="other" xlink:type="simple">Edwards, K. J., Wheat, C. G., Orcutt, B. N., Hulme, S., Becker, K., Jannasch, H., Haddad, A., Pettigrew, T., Rhinehart, W., Grigar, K., Bach, W., Kirkwood, W., and Klaus, A.: Design and deployment of borehole observatories and experiments during IODP Exp. 336, Mid-Atlantic Ridge flank at North Pond, in: Oric IODP 336, vol. Intergrated Ocean Drilling Program Management International, edited by: Edwards, K. J., Bach, W., Klaus, A., and the Expedition 336 Scientists Proceedings of the Integrated Ocean Drilling Program, Volume 336, Inc., Tokyo, 2012c.</mixed-citation>
</ref>
<ref id="ref9">
<label>9</label><mixed-citation publication-type="other" xlink:type="simple">Fisher, A. T.: Marine hydrogeology: recent accomplishments and future opportunities, Hydrogeol. J., 13, 69–97, 2005.</mixed-citation>
</ref>
<ref id="ref10">
<label>10</label><mixed-citation publication-type="other" xlink:type="simple">Fisk, M. R., Giovannoni, S. J., and Thorseth, I. H.: The extent of microbial life in volcanic crust of the ocean basins, Science, 281, 978–980, 1998.</mixed-citation>
</ref>
<ref id="ref11">
<label>11</label><mixed-citation publication-type="other" xlink:type="simple">Gieskes, J. M. and Magenheim, A. J.: Borehole fluid chemistry of DSDP Holes 395A and 534A, Geophys. Res. Lett., 19, 513–516, 1992.</mixed-citation>
</ref>
<ref id="ref12">
<label>12</label><mixed-citation publication-type="other" xlink:type="simple">Langseth, M. G., Becker, K., Von Herzen, R. P., and Schultheiss, P.: Heat and fluid flux through sediments on the western flank of the mid-Atlantic Ridge: a hydrogeological study of North Pond, Geophys. Res. Lett., 19, 517–520, 1992.</mixed-citation>
</ref>
<ref id="ref13">
<label>13</label><mixed-citation publication-type="other" xlink:type="simple">Melson, W. G., Rabinowitz, P. D., and et al.: Inital Reports of the Deep Sea Drilling Project, v 45. US Government Printing Office, Washington, D.C., 1978.</mixed-citation>
</ref>
<ref id="ref14">
<label>14</label><mixed-citation publication-type="other" xlink:type="simple">Orcutt, B., Wheat, C. G., and Edwards, K. J.: Subseafloor ocean crust microbial observatories: Development of FLOCS (FLow-through Osmo Colonization System) and evaluation of borehole construction methods, Geomicrobiol. J., 27, 143–157, 2010.</mixed-citation>
</ref>
<ref id="ref15">
<label>15</label><mixed-citation publication-type="other" xlink:type="simple">Orcutt, B. N., Bach, W., Becker, K., Fisher, A. T., Hentscher, M., Toner, B. M., Wheat, C. G., and Edwards, K. J.: Colonization of subsurface microbial observatories deployed in young ocean crust, The ISME Journal, 5, 692–703, &lt;a href=&quot;http://dx.doi.org/10.1038/ismej.2010.157&quot;&gt;https://doi.org/10.1038/ismej.2010.157&lt;/a&gt;, 2011.</mixed-citation>
</ref>
<ref id="ref16">
<label>16</label><mixed-citation publication-type="other" xlink:type="simple">Orcutt, B. N., Wheat, C. G., Hulme, S., Edwards, K. J., and Bach, W.: Oxygen consumption rates in subseafloor basaltic crust derived from a reaction transport model, Nature Communications, 4, 2539, &lt;a href=&quot;http://dx.doi.org/10.1038/ncomms3539&quot;&gt;https://doi.org/10.1038/ncomms3539&lt;/a&gt;, 2013.</mixed-citation>
</ref>
<ref id="ref17">
<label>17</label><mixed-citation publication-type="other" xlink:type="simple">Santelli, C. M., Orcutt, B. N., Banning, E., Bach, W., Moyer, C. L., Sogin, M. L., Staudigel, H., and Edwards, K. J.: Abundance and diversity of microbial life in ocean crust, Nature, 453, 653–656, 2008.</mixed-citation>
</ref>
<ref id="ref18">
<label>18</label><mixed-citation publication-type="other" xlink:type="simple">Santelli, C. M., Banerjee, N., Bach, W., and Edwards, K. J.: Tapping the Subsurface Ocean Crust Biosphere: Low Biomass and Drilling-Related Contamination Calls for Improved Quality Controls, Geomicrobiol. J., 27, 158–169, 2010.</mixed-citation>
</ref>
<ref id="ref19">
<label>19</label><mixed-citation publication-type="other" xlink:type="simple">Schmidt-Schierhorn, F., Kaul, N., Stephan, S., and Villinger, H.: Geophysical site survey results from North Pond (Mid-Atlantic Ridge), in: Proc IODP 336, vol. Intergrated Ocean Drilling Program Management International, Inc., edited by: Edwards, K. J., Bach, W., and Klaus, A., and Scientists E, Tokyo, 2012.</mixed-citation>
</ref>
<ref id="ref20">
<label>20</label><mixed-citation publication-type="other" xlink:type="simple">Staudigel, H., Furnes, H., McLoughlin, N., Banerjee, N. R., Connell, L. B., and Templeton, A.: 3.5 billion years of glass bioalteration: Volcanic rocks as a basis for microbial life?, Earth Sci. Rev., 89, 156–176, 2008.</mixed-citation>
</ref>
<ref id="ref21">
<label>21</label><mixed-citation publication-type="other" xlink:type="simple">Wheat, C. G., Edwards, K. J., Pettigrew, T., Jannasch, H. W., Becker, K., Davis, E. E., Villinger, H., and Bach, W.: CORK-Lite: Bringing Legacy Boreholes Back to Life, Sci. Drill., 14, 39–43, &lt;a href=&quot;http://dx.doi.org/10.2204/iodp.sd.14.05.2012&quot;&gt;https://doi.org/10.2204/iodp.sd.14.05.2012&lt;/a&gt;, 2012.</mixed-citation>
</ref>
</ref-list>
</back>
</article>