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  <front>
    <journal-meta>
<journal-id journal-id-type="publisher">SD</journal-id>
<journal-title-group>
<journal-title>Scientific Drilling</journal-title>
<abbrev-journal-title abbrev-type="publisher">SD</abbrev-journal-title>
<abbrev-journal-title abbrev-type="nlm-ta">Sci. Dril.</abbrev-journal-title>
</journal-title-group>
<issn pub-type="epub">1816-3459</issn>
<publisher><publisher-name>Copernicus GmbH</publisher-name>
<publisher-loc>Göttingen, Germany</publisher-loc>
</publisher>
</journal-meta>

    <article-meta>
      <article-id pub-id-type="doi">10.5194/sd-20-1-2015</article-id><title-group><article-title>IODP expedition 347: Baltic Sea basin paleoenvironment and biosphere</article-title>
      </title-group><?xmltex \runningtitle{IODP expedition 347: Baltic Sea basin paleoenvironment and biosphere}?><?xmltex \runningauthor{T.~Andr\'{e}n et al.}?>
      <contrib-group>
        <contrib contrib-type="author" corresp="yes" rid="aff1">
          <name><surname>Andrén</surname><given-names>T.</given-names></name>
          <email>thomas.andren@sh.se</email>
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff2">
          <name><surname>Barker Jørgensen</surname><given-names>B.</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff3">
          <name><surname>Cotterill</surname><given-names>C.</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff3">
          <name><surname>Green</surname><given-names>S.</given-names></name>
          
        <ext-link>https://orcid.org/0000-0002-7304-2821</ext-link></contrib>
        <contrib contrib-type="author" corresp="no" rid="aff4">
          <name><surname>the IODP expedition 347 scientific party</surname><given-names/></name>
          
        </contrib>
        <aff id="aff1"><label>1</label><institution>School of Natural Sciences, Technology and Environmental Studies
Södertörn University, Sweden</institution>
        </aff>
        <aff id="aff2"><label>2</label><institution>Center for Geomicrobiology
Department of Bioscience, Aarhus University, Denmark</institution>
        </aff>
        <aff id="aff3"><label>3</label><institution>British Geological Survey, Edinburgh, UK</institution>
        </aff>
        <aff id="aff4"><label>*</label><institution>A full list of authors and their affiliations appears at the end of the
paper.</institution>
        </aff>
      </contrib-group>
      <author-notes><corresp id="corr1">T. Andrén (thomas.andren@sh.se)</corresp></author-notes><pub-date><day>17</day><month>December</month><year>2015</year></pub-date>
      
      <volume>20</volume>
      <issue>20</issue>
      <fpage>1</fpage><lpage>12</lpage>
      <history>
        <date date-type="received"><day>27</day><month>September</month><year>2015</year></date>
           <date date-type="accepted"><day>23</day><month>November</month><year>2015</year></date>
      </history>
      <permissions>
<license license-type="open-access">
<license-p>This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">http://creativecommons.org/licenses/by/3.0/</ext-link></license-p>
</license>
</permissions><self-uri xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015.html">This article is available from https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015.html</self-uri>
<self-uri xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015.pdf">The full text article is available as a PDF file from https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015.pdf</self-uri>


      <abstract>
    <p>The Integrated Ocean Drilling Program (IODP) expedition 347 cored sediments from different settings of the Baltic
Sea covering the last glacial–interglacial cycle. The main aim was to
study the geological development of the Baltic Sea in relation to the
extreme climate variability of the region with changing ice cover and major
shifts in temperature, salinity, and biological communities. Using the
<italic>Greatship Manisha</italic> as a European Consortium for Ocean Research Drilling (ECORD) mission-specific platform, we recovered 1.6 km of core from nine
sites of which four were additionally cored for microbiology. The sites
covered the gateway to the North Sea and Atlantic Ocean, several sub-basins
in the southern Baltic Sea, a deep basin in the central Baltic Sea, and a river
estuary in the north.</p>
    <p>The waxing and waning of the Scandinavian ice sheet has profoundly affected
the Baltic Sea sediments. During the Weichselian, progressing glaciers
reshaped the submarine landscape and displaced sedimentary deposits from
earlier Quaternary time. As the glaciers retreated they left a complex
pattern of till, sand, and lacustrine clay, which in the basins has since been
covered by a thick deposit of Holocene, organic-rich clay. Due to the
stratified water column of the brackish Baltic Sea and the recurrent and
widespread anoxia, the deeper basins harbor laminated sediments that
provide a unique opportunity for high-resolution chronological studies.</p>
    <p>The Baltic Sea is a eutrophic intra-continental sea that is strongly
impacted by terrestrial runoff and nutrient fluxes. The Holocene deposits
are recorded today to be up to 50 m deep and geochemically affected by diagenetic
alterations driven by organic matter degradation. Many of the cored
sequences were highly supersaturated with respect to methane, which caused
strong degassing upon core recovery. The depth distributions of conservative
sea water ions still reflected the transition at the end of the last
glaciation from fresh-water clays to Holocene brackish mud. High-resolution
sampling and analyses of interstitial water chemistry revealed the
intensive mineralization and zonation of the predominant biogeochemical
processes. Quantification of microbial cells in the sediments yielded some
of the highest cell densities yet recorded by scientific drilling.</p>
  </abstract>
    </article-meta>
  </front>
<body>
      

<sec id="Ch1.S1" sec-type="intro">
  <title>Introduction</title>
      <p>The Baltic Sea basin (BSB) is one of the world's largest intra-continental
basins, occupying 373 000 km<inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msup></mml:math></inline-formula> and with a drainage area 4 times this
size (Fig. 1). Its mean depth is <inline-formula><mml:math display="inline"><mml:mo>∼</mml:mo></mml:math></inline-formula> 54 m, although a few
relatively deep basins exist (e.g., the eastern Gotland Basin, 248 m, and the
Landsort Deep, 459 m). The BSB has served as depositional sink throughout at
least the last during the last glacial cycle and its sediments comprise a
unique high-resolution archive of the paleoenvironmental history of the
large drainage area, the basin itself, and the neighboring gateways to the
North Sea. The location of the BSB in the heartland of the recurring
Scandinavian ice sheet (SIS) has resulted in a complex developmental
history, characteristic of many glaciated regions of the Northern
Hemisphere: repeated glaciations of different magnitudes, sensitive
responses to sea-level and gateway threshold changes, large shifts in
sedimentation patterns, and high sedimentation rates.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F1"><caption><p>Bathymetric map of the Baltic Sea with the nine coring sites,
M0059 to M0067.
Source: IODP Leg 347 expedition Report.</p></caption>
        <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015-f01.pdf"/>

      </fig>

      <p>The geographical position of the BSB makes it a unique link between the
Eurasian and the northwest European terrestrial records and as such also
serves as a link to the North Atlantic marine records and the Greenland ice
cores.</p>
      <p>The high sedimentation rates (100–500 cm/1000 years) of the BSB provide an
excellent opportunity to reconstruct in some parts climate variability of global
importance controlled by, for example, changes in meridional overturning circulation (MOC),
the North Atlantic Oscillation (NAO), and the Arctic oscillation (AO). These paleoenvironmental reconstructions offers a unique resolution from a
marine-brackish setting as some of the sediments can even be resolved on
inter-annual timescales. This makes the BSB highly suitable for sediment
coring from the last glacial cycle and a unique location to achieve
scientific objectives of high-resolution paleoceanography and paleoclimate
studies, as comparable sequences cannot be retrieved anywhere in the
surrounding onshore regions.</p>
</sec>
<sec id="Ch1.S2">
  <title>Glacial–interglacial history of the Baltic Sea basin</title>
      <p>The BSB has undergone many glaciations during the Quaternary. During the
last interglacial (the Eemian, marine isotope stage (MIS) 5e), the BSB was a larger and more saline
sea than the present Baltic Sea (Funder et al., 2002). Only fragments of the
Baltic glacial history are known. However, we do know that a Baltic glacial
event occurred during MIS 4 as recorded in sediments from northwest Finland
at <inline-formula><mml:math display="inline"><mml:mo>∼</mml:mo></mml:math></inline-formula> 64<inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula> N (Salonen et al., 2007). The first Baltic
ice lobe that advanced into Denmark is dated to
<inline-formula><mml:math display="inline"><mml:mo>∼</mml:mo></mml:math></inline-formula> 55–50 ky BP (thousand years before present; Houmark-Nielsen, 2007). From detailed
correlations and dating of southwestern Baltic glacial stratigraphies
(Houmark-Nielsen and Kjær, 2003), it is proposed that the southwestern
Baltic experienced two major ice advances during MIS 3, at <inline-formula><mml:math display="inline"><mml:mo>∼</mml:mo></mml:math></inline-formula> 50 and 30 ky BP.
However, the latter is highly debated (e.g.,
Kjellström et al., 2010). This enigmatic period between <inline-formula><mml:math display="inline"><mml:mo>∼</mml:mo></mml:math></inline-formula> 50 and 25 ky BP,
with its partly incompatible records, was followed by a
complex glaciation in the southern BSB (Houmark-Nielsen and Kjær, 2003)
leading up to the Last Glacial Maximum.</p>
      <p>Deglaciation of the southern BSB between 22 and 16 ky BP was complex, with
major deglacial phases interrupted by some intriguing SIS still stands and
re-advances (Houmark-Nielsen and Kjær, 2003; Larsen et al., 2009),
possibly as surges.</p>
      <p>Earlier studies have suggested that the Younger Dryas cold event (12.9 to
11.6 ky BP) was caused by freshwater runoff from the Laurentide (American)
ice sheet slowing the Atlantic MOC (Marshall
and Clark, 1999). However, state-of-the-art climate models require a
continuous freshwater forcing to produce millennia-long cold events (Liu et
al., 2009) and there are indications of substantial freshwater forcing from
the BSB predating the final drainage from the Baltic Ice Lake (e.g.,
Björck, 1995; Bennicke and Jensen, 2013).</p>
      <p>During the final drainage of the Baltic Ice Lake at <inline-formula><mml:math display="inline"><mml:mo>∼</mml:mo></mml:math></inline-formula> 11.7 ky BP
(Fig. 2a), almost 8000 km<inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">3</mml:mn></mml:msup></mml:math></inline-formula> of freshwater was released rapidly
into the North Atlantic (Jakobsson et al., 2007).</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F2"><caption><p><bold>(a)</bold> Estimated extension of the Scandinavian ice sheet at 11.7 ky BP just prior to the
final drainage of the Baltic Ice Lake. Dark blue: Atlantic Ocean, light
blue: Baltic Ice Lake. <bold>(b)</bold> Extension of the Littorina Sea during the time of maximum salinity in the
Baltic basin at ca. 6 ky BP. Source: Andrén et al. (2011).</p></caption>
        <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015-f02.jpg"/>

      </fig>

      <p>The next Baltic Sea stage, the Yoldia Sea, coincided with the onset of the
Holocene epoch (Walker et al., 2009) and the associated rapid warming. In
fact, the thicknesses of glacial varves in the northwestern Baltic proper
and <inline-formula><mml:math display="inline"><mml:mrow><mml:msup><mml:mi mathvariant="italic">δ</mml:mi><mml:mn>18</mml:mn></mml:msup></mml:mrow></mml:math></inline-formula>O values in ice cores from the Greenland Ice Core
Project (GRIP) display a noticeably similar pattern over a 150-year-long
transition period from Younger Dryas to pre-Boreal (Andrén et al., 1999,
2002). These records show a distinct increase in sedimentation rate as the
ice sheet began to melt and rapidly retreat. The following few hundred years
were characterized by rapid deglaciation of the SIS. Relative sea-level
lowering of the Yoldia Sea played an important role and were the result of a
combination of rapid regression in the recently deglaciated regions and
normal regression rates in southern Sweden (1.5–2 m/100 years).</p>
      <p>As the outlets to the west became shallower, subsequent damming forced the
water level inside the Baltic Basin to rise and the next stage began, the
Ancylus Lake. The sediments of this large freshwater lake generally contain
little organic material, which may be explained as a result of the large
meltwater inflow to the Baltic from the final deglaciation of the SIS and
also a result of erosion of the young soils from the recently deglaciated
drainage area. This created a lake environment with low nutrient input and
hence low productivity. The freshwater environment did not enable the
formation of a halocline but led to a well-mixed, oxygenated waterbody. The
relatively common sulfide-banded sediments of this stage can probably be
explained by later H<inline-formula><mml:math display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula>S diffusion from overlying, organic-rich Holocene
sediments (Sohlenius et al., 2001).</p>
      <p>The global melting of the large ice sheets over a couple of millennia caused
a 30 m rise in absolute sea level (Lambeck and Chappell, 2001). A
consequence of this was the flooding of the Öresund Strait, believed to
be the main gateway for the onset of the marine Littorina Sea stage. The
outlets/inlets through Öresund and Great Belt widened and became deeper,
resulting in greater water flow and gradually increasing salinity.</p>
      <p>The onset of the Littorina Sea stage can often be recognized as a marked
lithologic change in Baltic Sea sediment cores. The onset is represented by
a distinct increase in organic content and an increasing abundance of
brackish-marine diatoms (e.g., Sohlenius et al., 2001).</p>
      <p>Periods of deep-water hypoxia in the open Baltic Basin are evident in the
sediment record as extended sequences of laminated sediment. The Littorina
Sea (Fig. 2b) experienced a sustained period of elevated salinity and
resulting hypoxia between 8 and 4 ky BP (Zillén et al., 2008). After
<inline-formula><mml:math display="inline"><mml:mo>∼</mml:mo></mml:math></inline-formula> 4 ky BP, the salinity decreased gradually and oxygen
concentrations increased in the bottom waters.</p>
      <p>The human population growth and large-scale changes in land use that
occurred in the Baltic Sea watershed during the Medieval period between AD
750 and 1300 has been suggested as a triggering mechanism behind the
expansion of hypoxia that again occurred at this time (Zillén et al.,
2008). Alternative hypotheses suggest that the development of hypoxia in the
open Baltic Sea over the past 1000 years has mainly been driven by the climate
system (e.g., Kabel et al., 2012).</p>
</sec>
<sec id="Ch1.S3">
  <title>Scientific objectives</title>
      <p>The objectives of expedition 347 are categorized below.</p>
      <p>Geology and climate development objectives include the following:
<list list-type="bullet"><list-item><p>to increase our understanding of the climate system and the sea-level
dynamics of the last interglacial, including the climatic oscillations at the
transition between MIS 6 and MIS 5e, and in the initial, climatically oscillating
part of the last glacial (MIS 5d–5a);</p></list-item><list-item><p>to analyze environmental conditions during the warmest interval of MIS 5e
to elucidate possible future scenarios during warmer climate and higher sea-level
stands;</p></list-item><list-item><p>to evaluate how strongly the SIS responded, in time and space, to North
Atlantic climate forcing during the last glacial, and to what extent the dynamic
alterations of the SIS had an impact on the North Atlantic climate system;</p></list-item><list-item><p>to understand the feedbacks between the waterbody of the BSB, the SIS, and the North Atlantic
circulation;</p></list-item><list-item><p>to determine to what degree the glacier oscillations of the SIS margin
were synchronous on both sides of the main ice divide, centered along the Scandinavian
mountain chain, and whether the ice advances into the southern BSB can be recognized as large-scale
surges;</p></list-item><list-item><p>to describe how the highly oscillating climate pattern of MIS 3 is recorded
at the northeast margin of the North Atlantic in long and continuous sediment sequences in the
BSB;</p></list-item><list-item><p>to analyze if there are solar forcing signals in the melting record of
the shrinking ice sheet or in the precipitation-related fluvial system;</p></list-item><list-item><p>to reconstruct river discharge (and thereby also precipitation) with annual
resolution, several millennia back in time;</p></list-item><list-item><p>to determine how the in- and outflows to the Baltic Sea have varied over time,
and how this is related to changes in large-scale atmospheric circulation and sea level (threshold
depths);</p></list-item><list-item><p>to analyze how the general precipitation pattern, which is linked to the dominating
AO–NAO system over the northern circum-Atlantic and circum-Arctic region, change during the Holocene.</p></list-item></list></p>
      <p>Microbiology and geochemistry objectives include the following:
<list list-type="bullet"><list-item><p>to understand how microbial cell numbers are controlled in relation to depth,
age, lithology, and other environmental parameters in relatively young and organic-rich marine
sediments;</p></list-item><list-item><p>to determine the diversity and activity of subsurface microbial communities
through metagenomic, metatranscriptomic, and single-cell genomic analyses;</p></list-item><list-item><p>to determine whether the predominant microorganisms are selected by
environmental conditions prevailing in the subsurface today;</p></list-item><list-item><p>to determine whether the sub-seafloor microbial communities reflect past
environmental conditions, e.g., seawater or freshwater, cold or temperate climate, oxic or anoxic bottom
water;</p></list-item><list-item><p>to analyze whether viruses and unicellular eukaryotes play a role in the subsurface sediments;</p></list-item><list-item><p>to model and interpret past climate and paleoenvironment of the Baltic Sea basin from geochemical
proxies;</p></list-item><list-item><p>to analyze how the major glacial–interglacial shifts in the Baltic Sea affect sediment geochemistry
today;</p></list-item><list-item><p>to understand how nutrient loading, organic productivity and hypoxia have
varied in the Baltic Sea throughout the Holocene and what are the forcing factors.</p></list-item></list></p>
</sec>
<sec id="Ch1.S4">
  <title>Coring operations and strategy</title>
      <p>The drilling platform chosen for expedition 347 was the <italic>Greatship Manisha</italic>, an IMO (International Maritime Organization) Class II
dynamically positioned vessel with geotechnical coring capability (Fig. 3a).
The mobilization took place in Falmouth prior to transit to Kiel,
Germany, where the ESO (ECORD (European Consortium for Ocean Research Drilling) Science Operator) expedition project managers and offshore science
party participants boarded the vessel following a pre-cruise scientific
meeting in Copenhagen. Operations were conducted between 12 September and 1 November,
with demobilization of the vessel occurring back in
Falmouth on 5 and 6 November. In total, 37.1 days of
expedition 347 were spent operational on station, 8.5 days in transit
between sites, 1.3 days in port, 1.3 days on standby at station because of
weather, and 1.8 days on equipment-related downtime.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F3" specific-use="star"><caption><p><bold>(a)</bold> The geotechnical vessel, <italic>Greatship Manisha</italic> (of Singapore) equipped with a
drilling
derrick. <bold>(b)</bold> View from the derrick over the drill deck at mid-ship and the
“science garden” to the
aft. Image copyright ECORD/IODP.</p></caption>
        <?xmltex \igopts{width=341.433071pt}?><graphic xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015-f03.jpg"/>

      </fig>

      <p>The vessel was equipped with a large moon pool and Geoquip GMTR 120
heave-compensated derrick, with a 120 metric ton capacity top drive. Pipe
handling was carried out using a proprietary semiautomated handling system
utilizing a pipe handling crane with grab, a remotely operated iron
roughneck, and a proprietary catwalk system. A 4 m stroke passive heave
compensation (semiactive under development) was achieved using nitrogen gas
as a compensation buffer with Olmsted valve slingshot protection. The rig
was used in association with a 12 metric ton seabed template, fitted with
clamps and seabed transponder, to provide the reaction force for in-hole
tools. Wireline operation of the core barrel was conducted through the top
drive.</p>
      <p>Five methods of wireline coring were employed in addition to open-hole
drilling using a non-coring assembly. The primary coring tool was a piston corer system (PCS), operated by advancing the core barrel into the formation
through hydraulic pushing. However, where formation lithologies were either
unconsolidated, noncohesive, friable or very hard, an extended coring system (ECS), non-rotating core barrel system (NRCB), push coring assembly (PCA), or
hammer sampler (HS) were employed. The HS was the most rudimentary system
used during this expedition, consisting of a built-in hammer, which was
raised and lowered onto an anvil over a few meters distance. This tool was
used to obtain a spot sample when open-holing through coarser-grained
deposits, or when conventional coring methods had been unable to acquire a
sample. The non-coring assemble (NCA) used a Tricone Rock Roller drill bit
to plug the hole in the main core bit to advance without recovery.</p>
      <p>The core collected was 62 mm in diameter. The maximum core run length was
3.3 m. However, the length of a core run was chosen to maximize core
recovery and quality while maintaining hole stability, even at the expense
of overall penetration speed. When attempting to capture a lithologic
interface, as defined from seismic profiles, the run lengths were often
shortened by raising the corer above the bottom of the borehole by a known
height prior to pressuring the drill string. In some instances, the hole was
advanced by open holing – drilling ahead without recovering sediments.
This was done in difficult lithologies that could not be recovered
conventionally to enable recovery of other lithologies beneath these
intervals or when recovery (composite or from an individual hole) of an
interval had already reached &gt; 90 %, and the scientific
rationale was to try and get deeper within the time constraints. The advance
varied from a small offset of 0.5 m to ensure maximum core overlap between
holes in some locations to a more regular spacing of 3 m through the till
lithologies to monitor when or whether the lithology was changing.</p>
      <p>At the Ångermanälven River estuary site (M0061), there were initial
restrictions on coring the upper 50 cm of sediment due to potential heavy
metal contamination from industry. This was relaxed on assessment on-site.
At Hanö Bay, Bornholm basin, and Anholt loch sites (M0064, M0065, and
M0060,
respectively), downpipe camera or ROV  (remotely operated vehicle) surveys were conducted prior to
commencing coring, due to the potential for dumped WWII munitions on the
seabed. In addition, restrictions were placed on coring the upper 2m of
sediment at Bornholm Basin (M0065) due to the risk of dumped chemical
munitions.</p>
      <p>Offshore, the cores from paleo-oceanographic designated holes were carefully
curated by ESO staff before ephemeral physical (multi-sensor core logger,
MSCL) and geochemical properties were measured and preliminary core
catcher samples taken. Initial lithological and micropaleontological
descriptions were conducted by visual inspection through the liner and by using
core catcher materials. No further sampling, core splitting, or analysis work
was undertaken offshore.</p>
      <p>On microbiological designated holes, samples were taken on the drill floor
from the cut ends of core sections to capture ephemeral properties,
including methane gas and contamination tracer quantities. The cores were
then curated and run through a fast-track MSCL prior to extensive sampling
in the designated laboratory containers (Fig. 3b) for microbiological
research and for interstitial water analyses. In total &gt; 5800
samples were taken offshore.</p>
      <p>All cores, core catchers, headspace gas samples, interstitial water splits,
and digital data were transferred to the Integrated Ocean Drilling Program (IODP) core repository in Bremen,
Germany (BCR), at the end of the offshore phase. Prior to the start of the
Onshore Science Party (OSP), additional thermal conductivity measurements
and natural gamma radiation measurements were conducted on whole core
sections. The complete science party, as well as ESO and BCR personnel and student
helpers, met at the BCR from 22 January to 21 February to
split, analyze, and sample the cores according to standard IODP procedures,
and for post-cruise scientific research.</p>
</sec>
<sec id="Ch1.S5">
  <title>Preliminary expedition results</title>
      <p>In the following we present selected preliminary results from leg 347 on
lithology, biostratigraphy, dating, geochemistry, and microbiology. By these
chosen examples we briefly summarize how the expedition met some of the
objectives outlined above.</p><?xmltex \hack{\newpage}?>
<sec id="Ch1.S5.SS1">
  <title>Little Belt (sites M0059 and M0067) </title>
      <p>The lithology consists of diamicton interlayered with sand and silt in the
lower part of the core (Fig. 4a). These layers give OSL (optically stimulated luminescence) ages between ca. 362 ky BP at 146 m b.s.f.
(meters below seafloor) and ca. 103 ky BP
at 108 m b.s.f. This means that the sediments between these two levels
have recorded MIS 5. The recovered stratigraphy is, however, fragmented but
represents nonetheless a unique archive of a Pleistocene time window never
recovered before in the Baltic Sea. On top of this unit, at <inline-formula><mml:math display="inline"><mml:mo>∼</mml:mo></mml:math></inline-formula> 90–83 m b.s.f.,
is a 11 m thick sandy deposit with silt and clay laminas. The
OSL date from these sediments is ca. 44 ky BP.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F4" specific-use="star"><caption><p>Little Belt, site M0059. <bold>(a)</bold>Lithostratography with ages: green is ages derived from <inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mn>14</mml:mn></mml:msup></mml:math></inline-formula>C dating given in cal yr BP and red is ages derived from OSL dating given in ky BP. <bold>(b)</bold> Section
347-M0059A-16H-2 from
52 m b.s.f. shows the hiatus sand horizon of unit II at the transition from the
varved clay sequence
of Unit III into the laminated black organic-rich clay of subunit Ib. Inset:
close-up of the coarse-grained,
poorly sorted sand hiatus marking a low stand. <bold>(c)</bold> Relative distributions of
diatom taxa
according to their preferred habitat salinity. <bold>(d)</bold> Examples of interstitial
water chemistry:
diffraction-based salinity, alkalinity, and ammonium. Different symbols show
data from different
holes.</p></caption>
          <?xmltex \igopts{width=341.433071pt}?><graphic xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015-f04.jpg"/>

        </fig>

      <p>From 82 to 52 m b.s.f., glacial ice lake sediment is deposited as varved glacial
clay displaying thick varves in the lower part and thinner varves
deposited distal to the retreating ice margin in the upper part. This unit
is erosionally truncated and marked by a 2 cm thick sandy-silty layer at 52 m b.s.f.
displaying an upwards coarsening, which indicates deposition during a
rapid regression. The contact upwards to the overlaying gyttja clay is sharp
and possibly also erosional.</p>
      <p>At site M0059 we recovered 52 m of Holocene sediment with a
correspondingly high-mean sedimentation rate of 5–7 mm year<inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> based on four
radiocarbon dates received so far. The diatom flora in this unit of
organic-rich clay has recorded a sharp transition from freshwater to fully
marine conditions at ca. 48 m b.s.f., indicating a rapid marine transgression
(Fig. 4c). It should be noted, however, that final conclusions cannot be
drawn until the sequence has become more thoroughly analyzed.</p>
      <p>From site M0067 we only recovered ca. 4.4 m of Holocene mud on top
of medium to coarse sand interlayered with silt and clay, interpreted having
a glaciofluvial ice-proximal origin. There is obviously a large hiatus at
this site.</p>
      <p>The depth distributions of conservative seawater ions in the interstitial
water showed a distinct drop from seawater salinity in the Holocene sequence
to a minimum in salinity in the underlying glacial ice lake clay (Fig. 4d).
Interestingly, chloride-based salinity calculations did not show a broad
maximum at 10–30 m b.s.f. as did the diffraction-based salinity, which is
affected by all ions in the interstitial water. The difference is due to
very high DIC (dissolved inorganic carbon) and ammonium concentrations that add to the diffraction-based
salinity. These data reflect extremely high rates of organic matter
mineralization in the 50 m deep, organic-rich Holocene clay. Thus,
alkalinity values were up to 200 mM, which is probably a new IODP record,
while ammonium reached 40 mM.</p>
</sec>
<sec id="Ch1.S5.SS2">
  <title>Kattegat (site M0060)</title>
      <p>From site M0060 we cored a &gt; 200 m deep, almost continuous record
that consists of an 84 m diamict unit, in the lowermost part of the core. On
top of this unit alternating sandy and silty sequences are recorded with a
unit between 79 and 6 m b.s.f. that consists of varved glacial clay indicating
deposition in a glacial lake or marginal marine environment. It is possible
that this varved unit records freshwater outflow from the Baltic basin, and
it is therefore anticipated that it will give a yearly resolution.</p>
      <p>A gradually decreasing salinity was recorded down to ca. 125 m b.s.f. at this
site below, which the salinity increased distinctly again. Together with the
foraminifera and ostracode records this indicates a marine provenance of the
sediments below this level. Throughout the hole, cores also displayed
abundant snail and bivalve shells that will be useful for both dating and
environmental reconstruction.</p>
      <p>The sediment had surprisingly low mineralization rates with mainly sulfate
reduction and iron reduction as the dominant terminal processes of organic
matter oxidation. Methane appeared only at depths between 100 and 170 m b.s.f.
This methane is possibly not produced on site in the low-organic diamict
unit but may have been transported over a distance via the over-pressured
sandy aquifer.</p>
</sec>
<sec id="Ch1.S5.SS3">
  <?xmltex \opttitle{{\AA}ngerman\"{a}lven River estuary (sites M0061 and M0062)}?><title>Ångermanälven River estuary (sites M0061 and M0062)</title>
      <p>These two northernmost sites were selected as it was known that annual
varves have been deposited over several thousand years and are still being
formed at the mouth of the river. The many hundred years of varved sequences
collected at these sites will be correlated with and included in the so-called Swedish Time Scale, a continuous varve chronology covering the time
span from ca. 13 300 clay-varve years BP to the present.</p>
      <p>At both sites ca. 20 m of sediments were recovered, which displayed varves
that were 4–10 cm thick in the lower part and only a few millimeters thick in the
upper part. At site M0061 organic-rich mud in the uppermost 8 m displayed a
brackish influence that was evident from both the foraminifer and ostracode
fauna and from the diatom flora. It is noteworthy that this is the first
report of foraminifers and ostracodes in sediments of Holocene age this far
north in the Baltic Sea.</p>
</sec>
<sec id="Ch1.S5.SS4">
  <title>Landsort Deep (site M0063)</title>
      <p>In the Landsort Deep, which is the deepest basin in the Baltic Sea, we cored
to a depth of 95 m b.s.f. in a water depth of 437 m. At the deepest part between
95.8 and 93.4 m b.s.f. the sediment consists of a clast-poor sandy diamicton
overlaid by a 53 m thick unit of varved glacial clay. This uniquely long
varve sequence may enable a correlation to the Greenland ice core record
(cf. Andrén et al., 1999, 2002). The varves can possibly comprise as
much as 2,000 years in one continuous sedimentary record and may have
recorded, with annual resolution, the entire Younger Dryas as well as its
onset and termination (Fig. 5). In the upper part of this varved unit at
41.5 m b.s.f. a weak brackish influence is recorded in both the ostracode fauna
and in the diatom flora, which is interpreted as representing the short
brackish phase of the Yoldia Sea stage.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F5"><caption><p>Landsort Deep, site M0063. Lithostratigraphy with insets
<bold>(a)</bold> laminated Littorina Sea
mud (section 347-M0063C-12H-2) and <bold>(b)</bold> varved Baltic Ice Lake glacial clay
(section 347-M0063C-34H-2).</p></caption>
          <?xmltex \igopts{width=199.169291pt}?><graphic xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015-f05.jpg"/>

        </fig>

      <p>The overlaying grey homogenous clay converts gradually into a laminated
organic-rich mud and the first substantial inflows of marine water are
registered at 28 m b.s.f., as observed from a decrease in freshwater and
increase in brackish water diatom species and an increased abundance of
foraminifers. This first inflow is also registered as an increasing salinity
in the chemistry of the pore-water and probably represents the transition
from the lacustrine Ancylus Lake stage to the brackish-marine Littorina Sea
stage.</p>
      <p>The uppermost 28 m of the cored sediments consists of a laminated
organic-rich mud, sometimes referred to as clay gyttja in Baltic Sea
geological literature. This type of sediment indicates that the deepest part
of the Landsort Deep has been anoxic/hypoxic during the last ca. 7000 years.
So far we have only one <inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mn>14</mml:mn></mml:msup></mml:math></inline-formula>C date from this unit at 24.6 m b.s.f. yielding
an age of <inline-formula><mml:math display="inline"><mml:mrow><mml:mn>5850</mml:mn><mml:mo>±</mml:mo><mml:mn>120</mml:mn></mml:mrow></mml:math></inline-formula> cal yr BP.</p>
</sec>
<sec id="Ch1.S5.SS5">
  <?xmltex \opttitle{Han\"{o} Bay (site M0064)}?><title>Hanö Bay (site M0064)</title>
      <p>Four holes were drilled at this site of which three recovered an almost 30 m
long unit of clast-rich, stratified, muddy diamicton on top of a unit of
sandy clayey silt with thin intervals of diamicton. Varved glacial clay
deposited on top of this diamicton consisted of two different sub-units, a
lower dark-brownish-grey clay and an upper dark-greyish-brown clay separated
by a 2–3 cm thick organic-rich (LOI, loss on ignition; ca. 8 %) brown silty clay (84–87 cm in
section (b), Fig. 6). This unit has been <inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mn>14</mml:mn></mml:msup></mml:math></inline-formula>C dated in two cores and the
mean age of the unit is <inline-formula><mml:math display="inline"><mml:mrow><mml:mn>45 700</mml:mn><mml:mo>±</mml:mo><mml:mn>1500</mml:mn></mml:mrow></mml:math></inline-formula> cal yr BP, which means that this
lower varved clay sequence was deposited during a deglaciation much older
than the latest one.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F6"><caption><p>Hanö Bay, site M0064. Examples of varved glacial clay (<bold>a</bold> and
<bold>b</bold>) and diamicton <bold>(c, d)</bold>.
The greyish brown unit between 84 and 87 cm separating the two
different types of
varved glacial clays in section b have an age of 45 700 <inline-formula><mml:math display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 1500 cal yr BP.</p></caption>
          <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015-f06.jpg"/>

        </fig>

</sec>
<sec id="Ch1.S5.SS6">
  <title>Bornholm Basin (sites M0065 and M0066)</title>
      <p>The lithologies from these two sites are quite similar. The lowermost 40 m
of the stratigraphy consists of the same two different types of
varved glacial clays as at site M0064 although more expanded. Also at site
M0065 the two clay units are separated by a homogenous dark-grey layer, here
with a thickness of 60 cm, which probably corresponds to the dated layer at
site M0064.</p>
      <p>A shift from freshwater to brackish conditions is recorded in the diatom
flora at 10 m b.s.f. and most probably represents the Ancylus Lake–Littorina
Sea transition (Fig. 7). The lowermost part of this unit is laminated
whereas the upper 9 m is homogenous and contains abundant mollusk shell
fragments indicating well-oxygenated bottom water conditions.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F7"><caption><p>Bornholm Basin, site M0065. Relative distribution of diatom taxa
according to their
preferred habitat salinity.</p></caption>
          <?xmltex \igopts{width=184.942913pt}?><graphic xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015-f07.png"/>

        </fig>

<?xmltex \hack{\newpage}?>
</sec>
<sec id="Ch1.S5.SS7">
  <title>Microbiology</title>
      <p>Cell enumeration was done on board the <italic>Greatship Manisha</italic> by two different approaches. One was
the well-established epifluorescence counting of samples in which cells had
been DNA stained with the fluorescent dye, acridine orange. The other was
flow cytometry whereby cells were first extracted quantitatively from the
sediment and DNA stained with the fluorescent dye, SYBR (Synergy Brands Inc.) green. In the flow
cytometer, which was provided by the Kochi Core Repository, these cells were
counted automatically at a high rate, which increased the capacity for
onboard cell counts. Importantly, comparison of the two techniques provided
very similar cell numbers in most sediments studied. In some sediment
intervals, however, flow cytometry provided lower numbers than fluorescence
microscopy. This could be explained by incomplete detachment of cells from
exopolysaccharide particles, which prevented counting in the flow cytometer
but still enabled microscopic counting.</p>
      <p>Microbial cell abundances were extremely high at all sites studied, even
exceeding 10<inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mn>10</mml:mn></mml:msup></mml:math></inline-formula> cells cm<inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> in the Bornholm Basin, a new record for
the IODP. Cell numbers also remained surprisingly high at depth. This is in
accordance with the high organic carbon content in the Holocene deposits and
the high sedimentation rates. As an example from site M0059 in Little Belt,
the cell density was 10<inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">9</mml:mn></mml:msup></mml:math></inline-formula> cells cm<inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> at the sediment surface and
dropped only by 1 order of magnitude at the bottom of the Holocene at 50 m b.s.f.
where the sediment had an estimated age of &gt; 9000 years
(Fig. 8a). Cell numbers for site M0063 in the Landsort Deep (Fig. 8b)
provided another example of a good correspondence between the two
enumeration techniques in the lower part of the hole but showed an
increasing divergence towards the sediment surface due to the described
incomplete dispersion of cells.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F8" specific-use="star"><caption><p>Cell numbers from <bold>(a)</bold> Little Belt, site M0059, and <bold>(b)</bold> the Landsort
Deep, site M0063.
Red diamonds: cell counts by fluorescence microscopy (AODC, acridine orange direct counts); blue circles:
cell counts by
flow cytometry. The solid black line is a global regression line of
prokaryote cell numbers with
depth, while the dashed lines are upper and lower 95 % prediction limits
for the regression line
(Roussel et al., 2008).</p></caption>
          <?xmltex \igopts{width=426.791339pt}?><graphic xlink:href="https://sd.copernicus.org/articles/20/1/2015/sd-20-1-2015-f08.png"/>

        </fig>

      <p>Since it was not possible on this mission-specific platform to perform any
microbiological analyses other than cell counts, samples needed to be fixed
and/or frozen to be preserved for later studies in land-based laboratories.
Freezing may damage cell structures and can be a problem for studies that
depend on intact cells, such as fluorescence in situ hybridization (FISH or
CARD-FISH). However, a new CAS (Cells Alive System) freezer technique,
provided by the Kochi Core Repository, was available on board. By this
technique samples are cooled during exposure to a high-frequency alternating
electromagnetic field. When the temperature reaches <inline-formula><mml:math display="inline"><mml:mo>-</mml:mo></mml:math></inline-formula>10 <inline-formula><mml:math display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C the
electromagnetic field is switched off and freezing happens almost
instantaneously, which prevents the formation of ice crystals of a size that
damages cell structures.</p>
      <p>When drilling the holes for microbiology at four sites, contamination tests
were carried out (Smith et al., 2000; Lever et al., 2006). A perfluorocarbon
(PFC) tracer was pumped continuously into the drilling fluid and samples
were taken from three radial positions in each core immediately upon
recovery and cutting on the drill deck. As a new procedure for IODP, samples
were also taken routinely from fluid remaining in the core liner. Since this
represents the fluid to which the cores were exposed when taken in situ, these
fluid samples optimally represent the relevant contamination tracer
concentration. The results showed that the concentration of the tracer that
arrived at the bottom of the drill hole was mostly much lower than the
expected target concentration. The conclusion is that this sampling
procedure of liner fluid should be implemented on future IODP expeditions.
Also the land-based analytical procedure for PFC was improved relative to
IODP standard by heating samples while rotating before headspace sampling
and GC-ECD (gas chromatography with electron capture detector) analysis (M. A. Lever, unpublished). Furthermore, this procedure is
recommended for future deep biosphere expeditions when analyzing a PFC tracer.</p>
      <p>During expedition 347, a large number of samples were taken for many different
land-based research groups and for many different types of analysis. In
fact, the requested sampling frequency was so high in the upper sediment
intervals that most of the core was subsampled and it was occasionally
difficult to accommodate all the requests. In order to minimize the impact
of high levels of sampling, graphical sampling schemes were developed in
advance for each core. For the sampling of whole round cores, a small computer
program was developed to facilitate the sampling plan (Marshall, 2014). It
was also of great advantage on this expedition that samples for land-based
studies that relied on live microorganisms could be off-loaded from the
<italic>Greatship Manisha</italic> to a small boat and thereby arrived in the receiving laboratories in
Denmark, Germany, and the UK within a few days to a week after coring of each
microbiology hole.</p>
</sec>
</sec>
<sec id="Ch1.S6" sec-type="conclusions">
  <title>Conclusions and future work</title>
      <p>The OSL ages from site M0059 indicate that we reached at least some 350 ky
back in time. Without having all dates at hand, we assume that at site M0060
we recovered sediments dating back to the previous interglacial. This means
that the lower part of the holes from these two sites may cover the end of
MIS 6, MIS 5, and MIS 4, although with partly fragmented stratigraphies.</p>
      <p>Sediments of MIS 3 age have been recovered and dated at site M0059 and site
M0064 and are most probably also present in the sediments from site M0065
and perhaps also M0066. The recovered sediments, of pro-glacial sandur at
site M0059 and organic-bearing clay at site M0064 and M0065, indicate an
ice-free event in the middle of the Weichselian glacial with an age of <inline-formula><mml:math display="inline"><mml:mrow><mml:mn>44</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">4</mml:mn></mml:mrow></mml:math></inline-formula> ky BP.
Ongoing analyses of sediments from these sites may shed new light
on the history of the BSB and the behavior of the SIS during the enigmatic
MIS 3.</p>
      <p>Varved glacial clays deposited from the retreating SIS have been
recovered at all drilled sites, which makes environmental reconstructions
possible with annual resolution. Especially notable are the 52 m long varved
sequences from site M0063, possibly containing as much as 2000 varves and
probably covering the entire Younger Dryas. This unique varved sequence will
give us new insights into how the SIS responded to climate fluctuations,
including the slow cooling at the beginning and the rapid warming at the end
of the stadial. It will also give new information on the position of the ice
margin and thereby improve the correlation between ice margin positions in
eastern Sweden and southwestern Finland during the Younger Dryas.</p>
      <p>It should be noted that glacial varves have traditionally been assigned to
the latest deglaciation but the two units of varved glacial clay separated
by an organic-bearing clay with an age of 45 700 <inline-formula><mml:math display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 1500 cal yr BP
indicates a different scenario at site M0064. It is obvious that only the upper varved
sequence was deposited during the latest deglaciation whereas the lower
sequence represents an earlier deglaciation.</p>
      <p>Sediments of Holocene age have been recovered at most drilled sites but at
sites M0059 and M0063 the thickness of these sediments is spectacular, ca. 52 m
at site M0059 and ca. 45 m at site M0063. The extremely high
sedimentation rates at these two sites presented the possibility to reconstruct
the environmental response to climate warming leading up to the Holocene
thermal maximum, occurring some 8000 to 5000 years ago, with a resolution
never reached before in the Baltic Sea area. It will, for example, be
possible to detect small variations in, e.g., salinity, nutrient status,
temperature, ice cover, and periods of hypoxia/anoxia, possibly with an
annual resolution.</p>
      <p>Mission-specific platforms routinely work in shallow waters, and so
protocols are already in place to maximize the quality and amount of core
recovered when working in these environments, building on the knowledge
gained from four previous expeditions. However, the scope of the offshore
microbiological program was something not undertaken before on an MSP (mission-specific platforms).
Thanks to the early and extensive input and support from the microbiological
community, we were able to achieve a successful sampling campaign. However,
a detailed post-cruise review has shown areas in which additional protocols
need to be established in order to improve results in this discipline, e.g.,
the
addition of the contamination tracer to the drilling fluid at higher
concentrations.</p>
      <p>During expedition 347, four sites were selected for detailed studies of
geochemistry and microbiology. Nearly 6000 samples were taken offshore for
interstitial water analyses and microbiological studies. The many chemical
analyses done on board the <italic>Greatship Manisha</italic> and during the onshore phase have generated data
of outstanding quality and detail. The results provide novel insights into
the geochemical consequences of glacial to interglacial and fresh-water to marine
transitions combined with shifts from low-organic late-glacial clay to high
organic Holocene clay with extremely high sedimentation rates. These data
will be supplemented by a large diversity of inorganic, organic, and isotope
geochemical analyses in laboratories around the world.</p>
      <p>Detailed contamination tracer measurements were made during the expedition
with improved methods relative to previous IODP standards. Off-loading of
microbiology samples shortly after drilling enabled cultivation experiments
and studies of microbial activity to be done in land-based laboratories and
thereby overcome the limited laboratory facilities on the drillship. A
comparison of microbial cell counting techniques showed that flow cytometry
can provide data of equally good quality as microscopic counts and thereby
may enhance the capacity for cell quantification on future drilling
expeditions. A new CAS technology enables the freezing of microbiology
samples for sensitive post-cruise microbiology studies that require intact
DNA/RNA and cell membranes. A large number of samples are currently being
analyzed in many different laboratories and will provide metagenomic,
metatranscriptomic, and meta-metabolomic data as well as specific data on
microbial process rates, cultivation-based microbial diversity, and other
information for archaea, bacteria, and eukaryotic microorganisms as well as
viruses.</p>
      <p>Based on the preliminary lithological, biostratigraphic, paleomagnetic,
dating, geochemical, and microbiological data, we conclude that expedition
347 accomplished most of the objectives that we had outlined in IODP
proposal 672. This was indeed a successful expedition.</p>
      <p>The full expedition report is available for download from
<uri>http://publications.iodp.org/</uri>.</p>
<sec id="Ch1.S6.SSx1" specific-use="unnumbered">
  <title>Team members</title>
      <p>E. Andrén, J. Ash, T. Bauersachs, B. Cragg,
A.-S. Fanget, A. Fehr, W. Granoszewski, J. Groeneveld,
D. Hardisty, E. Herrero-Bervera,
O. Hyttinen, J. B. Jensen, S. Johnson, M. Kenzler, A. Kotilainen,
U. Kotthoff, I. P. G. Marshall, E. Martin, S. Obrochta, S. Passchier,
N. Quintana Krupinski, N. Riedinger,
C. Slomp, I. Snowball, A. Stepanova, S. Strano, A. Torti,
J. Warnock, N. Xiao, and R. Zhang.</p>
</sec>
</sec>

      
      </body>
    <back><ack><title>Acknowledgements</title><p>We thank the captain and crew of the <italic>Greatship Manisha</italic>, the operations superintendents and
all the expedition staff and technicians, whose outstanding professional
support ensured the successful drilling, sampling, and measurements on
expedition 347. We also thank the excellent management, staff and students
at the IODP Bremen Core Repository at MARUM, Bremen, for ensuring a very
effective and productive onshore sampling party. We thank the proponents and
other colleagues, who were engaged in the preparation of IODP proposal 672
for their great efforts that made this expedition happen.<?xmltex \hack{\newline}?><?xmltex \hack{\newline}?>
Edited by: U. Harms<?xmltex \hack{\newline}?>
Reviewed by: S. Björck and one anonymous referee</p></ack><ref-list>
    <title>References</title>

      <ref id="bib1.bib1"><label>1</label><mixed-citation>Andrén, T., Björck, J., and Johnsen, S.: Correlation of
Swedish glacial varves with the Greenland (GRIP) oxygen isotope record,
J. Quaternary Sci., 14, 361–371, <ext-link xlink:href="http://dx.doi.org/10.1002/(SICI)1099-1417(199907)14:4&lt;361::AID-JQS446&gt;3.0.CO;2-R" ext-link-type="DOI">10.1002/(SICI)1099-1417(199907)14:4&lt;361::AID-JQS446&gt;3.0.CO;2-R</ext-link>, 1999.</mixed-citation></ref>
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      <ref id="bib1.bib5"><label>5</label><mixed-citation>
Björck, S.: A review of the history of the Baltic Sea, 13.0–8.0 ka BP,
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  </ref-list><app-group content-type="float"><app><title/>

    </app></app-group></back>
    <!--<article-title-html>IODP expedition 347: Baltic Sea basin paleoenvironment and biosphere</article-title-html>
<abstract-html><h6 xmlns="http://www.w3.org/1999/xhtml" xmlns:m="http://www.w3.org/1998/Math/MathML" xmlns:svg="http://www.w3.org/2000/svg">Abstract. </h6><p xmlns="http://www.w3.org/1999/xhtml" xmlns:m="http://www.w3.org/1998/Math/MathML" xmlns:svg="http://www.w3.org/2000/svg" class="p">The Integrated Ocean Drilling Program (IODP) expedition 347 cored sediments from different settings of the Baltic
Sea covering the last glacial–interglacial cycle. The main aim was to
study the geological development of the Baltic Sea in relation to the
extreme climate variability of the region with changing ice cover and major
shifts in temperature, salinity, and biological communities. Using the
<span style="" class="text italic">Greatship Manisha</span> as a European Consortium for Ocean Research Drilling (ECORD) mission-specific platform, we recovered 1.6 km of core from nine
sites of which four were additionally cored for microbiology. The sites
covered the gateway to the North Sea and Atlantic Ocean, several sub-basins
in the southern Baltic Sea, a deep basin in the central Baltic Sea, and a river
estuary in the north.</p><p xmlns="http://www.w3.org/1999/xhtml" xmlns:m="http://www.w3.org/1998/Math/MathML" xmlns:svg="http://www.w3.org/2000/svg" class="p">The waxing and waning of the Scandinavian ice sheet has profoundly affected
the Baltic Sea sediments. During the Weichselian, progressing glaciers
reshaped the submarine landscape and displaced sedimentary deposits from
earlier Quaternary time. As the glaciers retreated they left a complex
pattern of till, sand, and lacustrine clay, which in the basins has since been
covered by a thick deposit of Holocene, organic-rich clay. Due to the
stratified water column of the brackish Baltic Sea and the recurrent and
widespread anoxia, the deeper basins harbor laminated sediments that
provide a unique opportunity for high-resolution chronological studies.</p><p xmlns="http://www.w3.org/1999/xhtml" xmlns:m="http://www.w3.org/1998/Math/MathML" xmlns:svg="http://www.w3.org/2000/svg" class="p">The Baltic Sea is a eutrophic intra-continental sea that is strongly
impacted by terrestrial runoff and nutrient fluxes. The Holocene deposits
are recorded today to be up to 50 m deep and geochemically affected by diagenetic
alterations driven by organic matter degradation. Many of the cored
sequences were highly supersaturated with respect to methane, which caused
strong degassing upon core recovery. The depth distributions of conservative
sea water ions still reflected the transition at the end of the last
glaciation from fresh-water clays to Holocene brackish mud. High-resolution
sampling and analyses of interstitial water chemistry revealed the
intensive mineralization and zonation of the predominant biogeochemical
processes. Quantification of microbial cells in the sediments yielded some
of the highest cell densities yet recorded by scientific drilling.</p></abstract-html>
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role of climate variability, environmental change and human impact, Earth Sci.
Rev., 91, 77–92, 2008.
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