Microbial diversity of drilling fluids from 3000 m deep Koyna pilot borehole provides insights into the deep biosphere of continental earth crust
Himadri Bose,Avishek Dutta,Ajoy Roy,Abhishek Gupta,Sourav Mukhopadhyay,Balaram Mohapatra,Jayeeta Sarkar,Sukanta Roy,Sufia K. Kazy,and Pinaki Sar
Himadri Bose
Environmental Microbiology and Genomics Laboratory, Department of
Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302,
West Bengal, India
Avishek Dutta
Environmental Microbiology and Genomics Laboratory, Department of
Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302,
West Bengal, India
Ajoy Roy
Department of Biotechnology, National Institute of Technology
Durgapur, Durgapur, 713209, West Bengal, India
Abhishek Gupta
Environmental Microbiology and Genomics Laboratory, Department of
Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302,
West Bengal, India
Sourav Mukhopadhyay
Environmental Microbiology and Genomics Laboratory, Department of
Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302,
West Bengal, India
Balaram Mohapatra
Environmental Microbiology and Genomics Laboratory, Department of
Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302,
West Bengal, India
Jayeeta Sarkar
Environmental Microbiology and Genomics Laboratory, Department of
Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302,
West Bengal, India
Environmental Microbiology and Genomics Laboratory, Department of
Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302,
West Bengal, India
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Drilling fluid (DF) used in the drilling of crystalline continental crust is considered a potent contaminant for subsurface rock samples, though it could provide a glimpse into the nature of deep subsurface life. Microbial communities of DF retrieved from Koyna pilot borehole (3000 m) in the Deccan Traps was explored through 16S rRNA and other diagnostic marker genes. Detection of extremophilic and other deep biosphere relevant microorganisms in DF redefined the role of DF in deep life research.
Drilling fluid (DF) used in the drilling of crystalline continental crust is considered a potent...