Workshop white paper
| 
29 May 2015
Workshop white paper |
| 29 May 2015
Workshop to develop deep-life continental scientific drilling projects
T. L. Kieft
CORRESPONDING AUTHOR
Department of Biology, New Mexico Tech, Socorro, NM 87801, USA
T. C. Onstott
Department of Geosciences, Princeton University, Princeton, NJ 08544, USA
L. Ahonen
Geological Survey of Finland, P.O. Box 96, 02151 Espoo, Finland
V. Aloisi
Laboratoire d'Océanographie Dynamique et de Climatologie, Université Pierre et Marie Curie, Place Jussieu 4, Case 100, 75252 Paris Cedex 05, France
F. S. Colwell
College of Earth, Ocean, and Atmospheric Sciences, Oregon State University, Corvallis, OR 97331, USA
B. Engelen
Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany
S. Fendrihan
Romanian Bioresources Centre, Sector 6, Bucharest, Romania
E. Gaidos
Dept. of Geology & Geophysics, University of Hawaii at Mānoa, Honolulu, HI 96822, USA
International Scientific Drilling Program, Helmholtz Centre Potsdam, GFZ German Research Centre for Geosciences, Telegrafenberg C-425, 14473 Potsdam, Germany
I. Head
Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne, NE17RU, UK
J. Kallmeyer
Helmholtz Centre Potsdam, GFZ German Research Centre for Geosciences, Telegrafenberg C-425, 14473 Potsdam, Germany
B. Kiel Reese
Department of Life Sciences, Texas A&M University-Corpus Christi, 6300 Ocean Drive, Unit 5800, Corpus Christi, TX 78412-5800, USA
L.-H. Lin
Department of Geosciences National Taiwan University, Taipei 106, Taiwan
P. E. Long
Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
D. P. Moser
Division of Earth and Ecosystem Sciences, Desert Research Institute, 755 E. Flamingo Road, Las Vegas, NV 89119, USA
H. Mills
Division of Natural Sciences, School of Science and Computer Engineering, University of Houston Clear Lake, Houston, TX 77058, USA
P. Sar
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721 302, India
D. Schulze-Makuch
Technical University Berlin, Berlin, Germany, and Washington State University, Pullman, WA 99164, USA
H. Stan-Lotter
Division of Molecular Biology, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria
D. Wagner
Helmholtz Centre Potsdam, GFZ German Research Centre for Geosciences, Telegrafenberg C-425, 14473 Potsdam, Germany
P.-L. Wang
Institute of Oceanography, National Taiwan University, Taipei 106, Taiwan
F. Westall
Centre de Biophysique Moléculaire, UPR CNRS 4301, rue Charles Sadron, 45071 Orléans Cedex 2, France
M. J. Wilkins
School of Earth Sciences and Department of Microbiology, The Ohio State University, Columbus, OH 43210, USA
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EGUsphere, https://doi.org/10.5194/egusphere-2025-2905, https://doi.org/10.5194/egusphere-2025-2905, 2025
This preprint is open for discussion and under review for Biogeosciences (BG).
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Bottom trawling is a fishing method that disturbs the seafloor and affects marine ecosystems. This study conducted experimental trawling and monitored biogeochemical changes over three weeks. Results showed reduced nutrient and alkalinity fluxes, decreased benthic carbon respiration, and disrupted biogeochemical processes. While the decline in alkalinity had only a minor effect on atmospheric CO2, the study highlights the lasting ecological impacts of bottom trawling.
Sara M. Defratyka, Julianne M. Fernandez, Getachew A. Adnew, Guannan Dong, Peter M. J. Douglas, Daniel L. Eldridge, Giuseppe Etiope, Thomas Giunta, Mojhgan A. Haghnegahdar, Alexander N. Hristov, Nicole Hultquist, Iñaki Vadillo, Josue Jautzy, Ji-Hyun Kim, Jabrane Labidi, Ellen Lalk, Wil Leavitt, Jiawen Li, Li-Hung Lin, Jiarui Liu, Lucia Ojeda, Shuhei Ono, Jeemin Rhim, Thomas Röckmann, Barbara Sherwood Lollar, Malavika Sivan, Jiayang Sun, Gregory T. Ventura, David T. Wang, Edward D. Young, Naizhong Zhang, and Tim Arnold
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Measurement of methane’s doubly substituted isotopologues at natural abundances holds promise for better constraining the Earth’s atmospheric CH4 budget. We compiled 1475 measurements from field samples and laboratory experiments, conducted since 2014, to facilitate the differentiation of CH4 formation pathways and processes, to identify existing gaps limiting application of Δ13CH3D and Δ12CH2D2, and to develop isotope ratio source signature inputs for global CH4 flux modelling.
Ellen Schnabel, Aurèle Vuillemin, Cédric C. Laczny, Benoit J. Kunath, André R. Soares, Alexander J. Probst, Rolando Di Primio, Jens Kallmeyer, and the PROSPECTOMICS Consortium
Biogeosciences, 22, 767–784, https://doi.org/10.5194/bg-22-767-2025, https://doi.org/10.5194/bg-22-767-2025, 2025
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This study analysed marine sediment samples from areas with and without minimal hydrocarbon seepage from reservoirs underneath. Depth profiles of dissolved chemical components in the pore water and molecular biological data revealed differences in microbial community composition and activity. These results indicate that even minor hydrocarbon seepage affects sedimentary biogeochemical cycling in marine sediments, potentially providing a new tool for the detection of hydrocarbon reservoirs.
Chueh-Chen Tung, Yu-Shih Lin, Jian-Xiang Liao, Tzu-Hsuan Tu, James T. Liu, Li-Hung Lin, Pei-Ling Wang, and Chih-Lin Wei
Biogeosciences, 21, 1729–1756, https://doi.org/10.5194/bg-21-1729-2024, https://doi.org/10.5194/bg-21-1729-2024, 2024
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This study contrasts seabed food webs between a river-fed, high-energy canyon and the nearby slope. We show higher organic carbon (OC) flows through the canyon than the slope. Bacteria dominated the canyon, while seabed fauna contributed more to the slope food web. Due to frequent perturbation, the canyon had a lower faunal stock and OC recycling. Only 4 % of the seabed OC flux enters the canyon food web, suggesting a significant role of the river-fed canyon in transporting OC to the deep sea.
George Westmeijer, Cristina Escudero, Claudia Bergin, Stephanie Turner, Magnus Ståhle, Maliheh Mehrshad, Prune Leroy, Moritz Buck, Pilar López-Hernández, Jens Kallmeyer, Ricardo Amils, Stefan Bertilsson, and Mark Dopson
Biogeosciences, 21, 591–604, https://doi.org/10.5194/bg-21-591-2024, https://doi.org/10.5194/bg-21-591-2024, 2024
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Rock cores down to 2250 m depth, groundwater-bearing fractures, and drilling fluid were sampled for DNA to characterize the subsurface microbial community. In general, microbial biomass was extremely low despite the employment of detection methods widespread in low-biomass environments. The described contamination control measures could support future sampling efforts, and our findings emphasize the use of sequencing extraction controls.
Steffen Kutterolf, Mark Brenner, Robert A. Dull, Armin Freundt, Jens Kallmeyer, Sebastian Krastel, Sergei Katsev, Elodie Lebas, Axel Meyer, Liseth Pérez, Juanita Rausch, Armando Saballos, Antje Schwalb, and Wilfried Strauch
Sci. Dril., 32, 73–84, https://doi.org/10.5194/sd-32-73-2023, https://doi.org/10.5194/sd-32-73-2023, 2023
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The NICA-BRIDGE workshop proposes a milestone-driven three-phase project to ICDP and later ICDP/IODP involving short- and long-core drilling in the Nicaraguan lakes and in the Pacific Sandino Basin to (1) reconstruct tropical climate and environmental changes and their external controlling mechanisms over several million years, (2) assess magnitudes and recurrence times of multiple natural hazards, and (3) provide
baselineenvironmental data for monitoring lake conditions.
Tomáš Fischer, Pavla Hrubcová, Torsten Dahm, Heiko Woith, Tomáš Vylita, Matthias Ohrnberger, Josef Vlček, Josef Horálek, Petr Dědeček, Martin Zimmer, Martin P. Lipus, Simona Pierdominici, Jens Kallmeyer, Frank Krüger, Katrin Hannemann, Michael Korn, Horst Kämpf, Thomas Reinsch, Jakub Klicpera, Daniel Vollmer, and Kyriaki Daskalopoulou
Sci. Dril., 31, 31–49, https://doi.org/10.5194/sd-31-31-2022, https://doi.org/10.5194/sd-31-31-2022, 2022
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The newly established geodynamic laboratory aims to develop modern, comprehensive, multiparameter observations at depth for studying earthquake swarms, crustal fluid flow, mantle-derived fluid degassing and processes of the deep biosphere. It is located in the West Bohemia–Vogtland (western Eger Rift) geodynamic region and comprises a set of five shallow boreholes with high-frequency 3-D seismic arrays as well as continuous real-time fluid monitoring at depth and the study of the deep biosphere.
Gilles Reverdin, Claire Waelbroeck, Catherine Pierre, Camille Akhoudas, Giovanni Aloisi, Marion Benetti, Bernard Bourlès, Magnus Danielsen, Jérôme Demange, Denis Diverrès, Jean-Claude Gascard, Marie-Noëlle Houssais, Hervé Le Goff, Pascale Lherminier, Claire Lo Monaco, Herlé Mercier, Nicolas Metzl, Simon Morisset, Aïcha Naamar, Thierry Reynaud, Jean-Baptiste Sallée, Virginie Thierry, Susan E. Hartman, Edward W. Mawji, Solveig Olafsdottir, Torsten Kanzow, Anton Velo, Antje Voelker, Igor Yashayaev, F. Alexander Haumann, Melanie J. Leng, Carol Arrowsmith, and Michael Meredith
Earth Syst. Sci. Data, 14, 2721–2735, https://doi.org/10.5194/essd-14-2721-2022, https://doi.org/10.5194/essd-14-2721-2022, 2022
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The CISE-LOCEAN seawater stable isotope dataset has close to 8000 data entries. The δ18O and δD isotopic data measured at LOCEAN have uncertainties of at most 0.05 ‰ and 0.25 ‰, respectively. Some data were adjusted to correct for evaporation. The internal consistency indicates that the data can be used to investigate time and space variability to within 0.03 ‰ and 0.15 ‰ in δ18O–δD17; comparisons with data analyzed in other institutions suggest larger differences with other datasets.
Henning Lorenz, Jan-Erik Rosberg, Christopher Juhlin, Iwona Klonowska, Rodolphe Lescoutre, George Westmeijer, Bjarne S. G. Almqvist, Mark Anderson, Stefan Bertilsson, Mark Dopson, Jens Kallmeyer, Jochem Kück, Oliver Lehnert, Luca Menegon, Christophe Pascal, Simon Rejkjær, and Nick N. W. Roberts
Sci. Dril., 30, 43–57, https://doi.org/10.5194/sd-30-43-2022, https://doi.org/10.5194/sd-30-43-2022, 2022
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The Collisional Orogeny in the Scandinavian Caledonides project provides insights into the deep structure and bedrock of a ca. 400 Ma old major orogen to study deformation processes that are hidden at depth from direct access in modern mountain belts. This paper describes the successful operations at the second site. It provides an overview of the retrieved geological section that differs from the expected and summarises the scientific potential of the accomplished data sets and drill core.
Tzu-Hsuan Tu, Li-Ling Chen, Yi-Ping Chiu, Li-Hung Lin, Li-Wei Wu, Francesco Italiano, J. Bruce H. Shyu, Seyed Naser Raisossadat, and Pei-Ling Wang
Biogeosciences, 19, 831–843, https://doi.org/10.5194/bg-19-831-2022, https://doi.org/10.5194/bg-19-831-2022, 2022
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This investigation of microbial biogeography in terrestrial mud volcanoes (MVs) covers study sites over a geographic distance of up to 10 000 km across the Eurasian continent. It compares microbial community compositions' coupling with geochemical data across a 3D space. We demonstrate that stochastic processes operating at continental scales and environmental filtering at local scales drive the formation of patchy habitats and the pattern of diversification for microbes in terrestrial MVs.
Ulrich Harms, Ulli Raschke, Flavio S. Anselmetti, Michael Strasser, Volker Wittig, Martin Wessels, Sebastian Schaller, Stefano C. Fabbri, Richard Niederreiter, and Antje Schwalb
Sci. Dril., 28, 29–41, https://doi.org/10.5194/sd-28-29-2020, https://doi.org/10.5194/sd-28-29-2020, 2020
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Hipercorig is a new modular lake sediment coring instrument based on a barge and a hydraulic corer system driven by a down-the-hole hammer. Hipercorig's performance was tested on the two periglacial lakes, namely Mondsee and Constance, located on the northern edge of the Alpine chain. Up to 63 m of Holocene lake sediments and older meltwater deposits from the last deglaciation were recovered for the first time.
Aurèle Vuillemin, André Friese, Richard Wirth, Jan A. Schuessler, Anja M. Schleicher, Helga Kemnitz, Andreas Lücke, Kohen W. Bauer, Sulung Nomosatryo, Friedhelm von Blanckenburg, Rachel Simister, Luis G. Ordoñez, Daniel Ariztegui, Cynthia Henny, James M. Russell, Satria Bijaksana, Hendrik Vogel, Sean A. Crowe, Jens Kallmeyer, and the Towuti Drilling Project
Science team
Biogeosciences, 17, 1955–1973, https://doi.org/10.5194/bg-17-1955-2020, https://doi.org/10.5194/bg-17-1955-2020, 2020
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Ferruginous lakes experience restricted primary production due to phosphorus trapping by ferric iron oxides under oxic conditions. We report the presence of large crystals of vivianite, a ferrous iron phosphate, in sediments from Lake Towuti, Indonesia. We address processes of P retention linked to diagenesis of iron phases. Vivianite crystals had light Fe2+ isotope signatures and contained mineral inclusions consistent with antecedent processes of microbial sulfate and iron reduction.
Julia Mitzscherling, Fabian Horn, Maria Winterfeld, Linda Mahler, Jens Kallmeyer, Pier P. Overduin, Lutz Schirrmeister, Matthias Winkel, Mikhail N. Grigoriev, Dirk Wagner, and Susanne Liebner
Biogeosciences, 16, 3941–3958, https://doi.org/10.5194/bg-16-3941-2019, https://doi.org/10.5194/bg-16-3941-2019, 2019
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Permafrost temperatures increased substantially at a global scale, potentially altering microbial assemblages involved in carbon mobilization before permafrost thaws. We used Arctic Shelf submarine permafrost as a natural laboratory to investigate the microbial response to long-term permafrost warming. Our work shows that millennia after permafrost warming by > 10 °C, microbial community composition and population size reflect the paleoenvironment rather than a direct effect through warming.
Laura Perrin, Ian Probert, Gerald Langer, and Giovanni Aloisi
Biogeosciences, 13, 5983–6001, https://doi.org/10.5194/bg-13-5983-2016, https://doi.org/10.5194/bg-13-5983-2016, 2016
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Coccolithophores are calcifying marine algae that play an important role in the oceanic carbon cycle. Deep niches of coccolithophores exist in the ocean and are poorly understood. Laboratory cultures with the coccolithophore Emiliania huxleyi were carried out to reproduce the environmental conditions (light–nutrient limitation) of a deep niche in the South Pacific Ocean. Physiological modelling of experimental results allows us to estimate the growth rates of coccolithophores in this niche.
Anh Phuong Tran, Baptiste Dafflon, Susan S. Hubbard, Michael B. Kowalsky, Philip Long, Tetsu K. Tokunaga, and Kenneth H. Williams
Hydrol. Earth Syst. Sci., 20, 3477–3491, https://doi.org/10.5194/hess-20-3477-2016, https://doi.org/10.5194/hess-20-3477-2016, 2016
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Quantifying water and heat fluxes in the shallow subsurface is particularly important due to their strong control on recharge, evaporation and biogeochemical processes. This study developed and tested a new inversion scheme to estimate subsurface hydro-thermal parameters by joint using different hydrological, thermal and geophysical data. It is especially useful for the increasing number of studies that are taking advantage of autonomously collected measurements to explore ecosystem dynamics.
James M. Russell, Satria Bijaksana, Hendrik Vogel, Martin Melles, Jens Kallmeyer, Daniel Ariztegui, Sean Crowe, Silvia Fajar, Abdul Hafidz, Doug Haffner, Ascelina Hasberg, Sarah Ivory, Christopher Kelly, John King, Kartika Kirana, Marina Morlock, Anders Noren, Ryan O'Grady, Luis Ordonez, Janelle Stevenson, Thomas von Rintelen, Aurele Vuillemin, Ian Watkinson, Nigel Wattrus, Satrio Wicaksono, Thomas Wonik, Kohen Bauer, Alan Deino, André Friese, Cynthia Henny, Imran, Ristiyanti Marwoto, La Ode Ngkoimani, Sulung Nomosatryo, La Ode Safiuddin, Rachel Simister, and Gerald Tamuntuan
Sci. Dril., 21, 29–40, https://doi.org/10.5194/sd-21-29-2016, https://doi.org/10.5194/sd-21-29-2016, 2016
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The Towuti Drilling Project seeks to understand the long-term environmental and climatic history of the tropical western Pacific and to discover the unique microbes that live in metal-rich sediments. To accomplish these goals, in 2015 we carried out a scientific drilling project on Lake Towuti, located in central Indonesia. We recovered over 1000 m of core, and our deepest core extended 175 m below the lake floor and gives us a complete record of the lake.
Lotta Purkamo, Malin Bomberg, Riikka Kietäväinen, Heikki Salavirta, Mari Nyyssönen, Maija Nuppunen-Puputti, Lasse Ahonen, Ilmo Kukkonen, and Merja Itävaara
Biogeosciences, 13, 3091–3108, https://doi.org/10.5194/bg-13-3091-2016, https://doi.org/10.5194/bg-13-3091-2016, 2016
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The microbial communities of up to 2.3 km depth of Precambrian crystalline bedrock fractures share features with serpenization-driven microbial communities in alkaline springs and subsurface aquifers. This study suggests that phylotypes belonging to Burkholderiales and Clostridia are possible "keystone microbial species" in Outokumpu deep biosphere. Many of the keystone species belong to the rare biosphere with low abundance but a wide range of carbon substrates and a capacity for H2 oxidation.
H. J. Mills, J. de Leeuw, K.-U. Hinrichs, F. Inagaki, and J. Kallmeyer
Sci. Dril., 20, 59–65, https://doi.org/10.5194/sd-20-59-2015, https://doi.org/10.5194/sd-20-59-2015, 2015
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Proceedings and results are presented from the Seoul 2014 Advancing Subsurface Biosphere and Paleoclimate Research workshop. Participants discussed past and present directions of IODP and ICDP subsurface research, including efforts with DCO and IMPRESS. Discussions led to the formation of a level-based communication system with the goal of improving communication and expectations between all drilling disciplines. The production of a biology-themed handbook to guide surface research is planned.
G. Aloisi
Biogeosciences, 12, 4665–4692, https://doi.org/10.5194/bg-12-4665-2015, https://doi.org/10.5194/bg-12-4665-2015, 2015
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Metabolic rates and cell size in coccolithophore algae covary consistently in a large number of separate culture experiments as temperature, irradiance, nutrient and pCO2 conditions change.
These changes are comparable to the changes in cell size observed in the natural environment, both in the modern ocean and in marine sediments.
Changes in coccolithophore cell size in the field will help in understanding how this key phytoplankton species reacts to climate change.
B. N. Orcutt, D. E. LaRowe, K. G. Lloyd, H. Mills, W. Orsi, B. K. Reese, J. Sauvage, J. A. Huber, and J. Amend
Sci. Dril., 17, 61–66, https://doi.org/10.5194/sd-17-61-2014, https://doi.org/10.5194/sd-17-61-2014, 2014
Related subject area
Location/Setting: Continental | Subject: Microbiology | Geoprocesses: Deep biosphere
Microbial diversity of drilling fluids from 3000 m deep Koyna pilot borehole provides insights into the deep biosphere of continental earth crust
Drilling into an active mofette: pilot-hole study of the impact of CO2-rich mantle-derived fluids on the geo–bio interaction in the western Eger Rift (Czech Republic)
Himadri Bose, Avishek Dutta, Ajoy Roy, Abhishek Gupta, Sourav Mukhopadhyay, Balaram Mohapatra, Jayeeta Sarkar, Sukanta Roy, Sufia K. Kazy, and Pinaki Sar
Sci. Dril., 27, 1–23, https://doi.org/10.5194/sd-27-1-2020, https://doi.org/10.5194/sd-27-1-2020, 2020
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Drilling fluid (DF) used in the drilling of crystalline continental crust is considered a potent contaminant for subsurface rock samples, though it could provide a glimpse into the nature of deep subsurface life. Microbial communities of DF retrieved from Koyna pilot borehole (3000 m) in the Deccan Traps was explored through 16S rRNA and other diagnostic marker genes. Detection of extremophilic and other deep biosphere relevant microorganisms in DF redefined the role of DF in deep life research.
Robert Bussert, Horst Kämpf, Christina Flechsig, Katja Hesse, Tobias Nickschick, Qi Liu, Josefine Umlauft, Tomáš Vylita, Dirk Wagner, Thomas Wonik, Hortencia Estrella Flores, and Mashal Alawi
Sci. Dril., 23, 13–27, https://doi.org/10.5194/sd-23-13-2017, https://doi.org/10.5194/sd-23-13-2017, 2017
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